The purpose of this study was to document changes in strength, body composition, and blood lipid profiles in sedentary, overweight, hypercholesterolemic male subjects who participated in a 12-week resistance training program and who supplemented their usual diets with either whey or soy protein

versus placebo. It was hypothesized that: 1) subjects receiving either protein supplement would have equivalent gains in both strength and lean body mass and these gains would be greater than the placebo group; 2) Subjects receiving the soy supplementation would have a significant reduction in fasting blood lipid levels versus the whey and placebo groups. Methods Subjects Thirty two healthy males from the Western New GDC-0449 order York community volunteered to participate in the study. These men (age range 21–50 years; mean 38) were generally sedentary, overweight IWP-2 in vivo [BMI (body mass index) 25.0–29.9], with mild to

moderate hypercholesterolemia, but otherwise in overall good health. Inclusion criteria of a general sedentary lifestyle ensured that no participant recorded a BMI above 25.0 due to significant muscle mass at the beginning of the study period. Each subject was informed of the purpose and procedures of the study, and provided informed consent in accordance with the Human Subject’s Review Committee of the University at Buffalo. Criteria for inclusion were: sedentary lifestyle (none or minimal routinely planned physical activity); BMI between 25.0–29.9; normal fasting blood glucose; and two or more of the following CVD risk factors: total cholesterol 200–240 mg/dl,

LDL cholesterol 130–160 mg/dl, or triglycerides 150–200 mg/dl. Exclusion criteria included any prior cerebrovascular event that required hospitalization or surgery, habitual soy consumers, smokers, Phospholipase D1 orthopedic or neuromuscular disorders that precluded participation in resistive exercise training and medications that affect lipid metabolism, blood pressure or cardiac function. Anthropometrics Each subject’s height was measured using a stadiometer (Perspective Enterprises, Kalamazoo, Michigan) and body mass was measured on a Health-O-Meter scale (Bridgeview, Illinois). Skinfolds (tricep, supraillium, abdomen and thigh) were measured with Lange skinfold calipers (Cambridge Scientific Industries, Inc., Cambridge, Maryland). All skinfolds were measured by the same investigator utilizing the same caliper for each study subject. Measures were taken in triplicate with a 2 mm reliability range. Final skinfolds were taken without viewing initial measures to minimize experimentation bias. Percent body fat was then estimated using the 4-site formula from ACSM’s Resource Manual for Guidelines for Testing and Prescription [22].