Of 44 cryptogenic Selleckchem Navitoclax cirrhosis patients who underwent liver biopsy, 17 (39%) had NCIPH and 8 had “true cryptogenic cirrhosis.” NCIPH and true cryptogenic cirrhosis patients were 27 (range, 14-59) and 42 (range, 25-67) years old, respectively; 10 and 4 patients, respectively, were males. Hepatic venous pressure gradient measured in 15 NCIPH and 4 true cryptogenic cirrhosis patients was 7 (range, 1-21) and

18 (range, 10-27) mmHg, respectively (P = 0.012). Liver biopsies were performed percutaneously in 4 NCIPH patients and transjugularly in 13. Number of cores in percutaneous biopsies was 3 per patient and 3 (range, 1-6) in transjugular biopsies; length of the largest core was 13 (range,12-15) in percutaneous and 12 mm (range, 6-16) in transjugular biopsies. The number of portal tracts in liver biopsies was 10 (range, 5-20). Liver biopsies showed no significant fibrosis (6 patients), mild portal/periportal fibrosis (10), moderate fibrosis (1), mild perisinusoidal fibrosis (1), abnormal portal venous ectasia (6), and mild diffuse sinusoidal dilatation (8); no patient had cirrhosis or severe fibrosis. In summary, in 2009-2010 and 2005-2007,4

39%-48% of patients with clinical diagnosis of cryptogenic cirrhosis who underwent liver biopsy at our center had NCIPH. Ashish Goel*, Banumathi Ramakrishna†, Kadiyala Madhu*, Uday Zachariah*, Jeyamani Ramachandran*, Shyamkumar N. Keshava‡, Elwyn Elias§, Chundamannil E. Eapen*, * Departments of Hepatology, Christian Medical College, Vellore, India, † Pathology, Christian selleck screening library Medical JQ1 College, Vellore,

India, ‡ Radiology, Christian Medical College, Vellore, India, § Liver Unit, University Hospital Birmingham , Birmingham, United Kingdom. “
“Background and Aim:  The major transporter responsible for bile acid uptake from the intestinal lumen is the apical sodium-dependent bile acid transporter (ASBT, SLC10A2). Analysis of the SLC10A2 gene has identified a variety of sequence variants including coding region single nucleotide polymorphisms (SNPs) that may influence bile acid homeostasis/intestinal function. In this study, we systematically characterized the effect of coding SNPs on SLC10A2 protein expression and bile acid transport activity. Methods:  Single nucleotide polymorphisms in SLC10A2 from genomic DNA of ethnically-defined healthy individuals were identified using a polymerase chain reaction (PCR)-based temperature gradient capillary electrophoresis (TGCE) system. A heterologous gene expression system was used to assess transport activity of SLC10A2 nonsynonymous variants and missense mutations. Total and cell surface protein expression of wild-type and variant ASBT was assessed by Western blot analysis and immunofluorescence confocal microscopy. Expression of ASBT mRNA and protein was also measured in human intestinal samples.