The loss of HDAC1 and/or HDAC2 (HDAC1/2) protein resulted in impaired liver regeneration. HDAC1/2 inactivation did not decrease hepatocytic 5-bromo-2-deoxyuridine uptake or the expression of proliferating cell nuclear antigen, cyclins, or cyclin-dependent
kinases. However, the levels of Ki67, a mitotic marker that is expressed from the mid-G1 phase to the end of mitosis and is closely involved in the regulation of mitotic progression, were greatly decreased, and abnormal mitosis lacking Ki67 expression was frequently observed in HDAC1/2-deficient livers. The down-regulation of either HDAC1/2 or Ki67 in the mouse liver cancer cell line Hepa1-6 resulted in similar mitotic defects. Finally, both HDAC1 and HDAC2 proteins were associated with the Ki67 gene mediated by CCAAT/enhancer-binding protein http://www.selleckchem.com/products/PLX-4032.html β. Conclusion: Both HDAC1 and HDAC2 play crucial roles in the regulation of liver regeneration. The loss of HDAC1/2 inhibits Ki67 expression
and Erlotinib price results in defective hepatocyte mitosis and impaired liver regeneration. (Hepatology 2013; 58:2089–2098) Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from specific lysine residues on core histones and thereby regulate gene transcription through the structural modification of histones and chromatin.[1, 2] HDACs are recruited to multiprotein complexes on the genome and serve as epigenetic corepressors to facilitate the inhibition of target gene transcription; in this way, they regulate many physiological processes, including mitosis, apoptosis, and tumorigenesis.[3-5] The deregulation of HDACs is often associated with the development and progression of various cancers, and a number of HDAC inhibitors (HDACis) are currently being investigated for use in clinical tumor therapy.[6, 7] HDAC1 and HDAC2, the two members of the class I HDAC family, are ubiquitously expressed in organs and tissues, including the liver.[8]
Similar to other HDACs, neither bind directly to DNA; instead, Calpain HDAC1 and HDAC2 typically associate with corepressors, such as Sin3-SAP, NuRD, and CoREST, to form transcriptional corepressor complexes.[9] HDAC1 and/or HDAC2 (HDAC1/2) are also required for chromatin condensation, spindle formation, and chromosome separation during the mitotic phase of the cell cycle, and HDAC1/2 deregulation can lead to abnormal mitosis.[10-12] Most of the current knowledge regarding the role of HDAC1/2 has come from cancer research. A number of studies have used HDACis or small interfering RNA (siRNA) to investigate the role of HDAC1/2 in cell proliferation both in vivo and in vitro.