Results: From 1127 selleck screening library patients we found that Helicobacter pylori positive from histopathology
were 134 patient and 993 patients were negative for Helicobacter (11.8%). The proportion of male was 55.3% and female was 44.7%. Mean age from patients was 47 years old. Mean age for metaplasia patients was 55 years old, dysplasia patients was 73 years old and malignant patients was 64 years old. From this study we found metaplasia from 45 patients, dysplasia 4 patients and 7 patients has gastric malignancy. Metaplasia was found 33 from 955 (3, 3%) who were negative for H. pylori and 12 patients from 116 (9, 2%) who were positive for H. pylori. From seven patients with gastric malignancy were positive for Helicobacter infection. Conclusion: From this study we found that a Helicobacter pylori infection was associated with metaplasia intestinal and gastric malignancy.
Key Word(s): 1. H. pylori infection; 2. Jakarta; 3. gastric metaplasia; 4. gastric malignancy; Presenting Author: NIAN FANG Additional Authors: HUIQING ZHANG, NIXIAO HAN, GEN HUANG, LINGZI FANG, NONGRONG WANG, KUNHE ZHANG Corresponding Author: NIAN FANG Affiliations: selleck chemicals university Objective: To observe the effects of AFP gene silencing by siRNA on Survivin mRNA of hepatocellular carcinoma cell line HepG2. Methods: AFP gene expression was downregulated in HepG2 cell by RNAi, and the AFP content in supernatant was detected by ELISA, Survivin mRNA level was tested by RT-PCR. MTT was applied to evaluated cell proliferation, and flow cytometry was employed MCE to observe cell apoptosis. Results: At the time of 48 hours after transfection, AFP expression was almost completely inhibited, cell proliferation activity was decreased 43.1%, cell apoptosis rate was increased
24.3%, and the Survivin mRNA expression was reduced to 22.0% in the experimental group, but no obvious changes were observed in the negative control and blank groups. Conclusion: AFP gene silenced by RNAi induces growth inhibition and apoptosis promotion of hepatocellular carcinoma cell line HepG2, and it maybe associated with the suppression of Survivin mRNA. Key Word(s): 1. HCC; 2. AFP; 3. Survivin; 4. RNA interference; Presenting Author: PING HAN Additional Authors: WEI YAN, DEAN TIAN Corresponding Author: PING HAN, WEI YAN, DEAN TIAN Affiliations: Tongji Medical College Objective: Epithelial-mesenchymal transition (EMT) is a critical step in the metastatic progression of epithelial carcinomas, Blood vessel epicardial substance (BVES) was found to prevent migration and invasion in some solid cancers, however, the role it plays in human hepatocellular carcinoma (HCC) has never been detailed researched. Netrin-1 is a secreted, laminin-related protein which was discovered to promote EMT of HCC in our previous research. In this study, we aimed to exam the role of BVES in HCC, and investigate the upstream factor Netrin-1 role in regulating the expression of BVES.