Methods Subjects Two groups of students

from 17 to 21 years old were enlisted. Each group had 10 students. The first group (i.e., lowlanders) had students aged between 17 and 19 who were natives of Yunnan province, China, living continuously at 1700 m above sea level. The second group (i.e., highlanders) consisted of students aged between 17 and Inhibitors,research,lifescience,medical 21 who were dwelling at 3000 m or more above sea level in the highlands. The high throughput screening compounds latter group of students had come to Yunnan as students in the university just 1 month prior to this study. The project had informed consent from all the students involved and had ethical approval from the hospital and university involved. Simple mental task of mathematics The students were asked to compute a short and easy mathematical question by heart after presented the question via a slide.

The simple question was in the form of X × Y + Z. While the students were computing, fMRI was performed on their brains. Apart from the lowlander and Inhibitors,research,lifescience,medical the highlander groups, five controls (age matched) were employed. Inhibitors,research,lifescience,medical These latter subjects were provided with slides of different sceneries while fMRI were performed on them. Image processing Processing and analysis of fMRI data was performed using the MATLAB software coupled with the Statistical Parametric Mapping 8 (SPM8) method developed by the Wellcome Department of Cognitive Neurology, University College London (http://www.fil.ion.ucl.ac.uk/spm/) as described previously (Yu et al. 2012). The steps are described briefly as follows. Images from each subject were realigned

with the first scanned image to correct for head movement artefacts during the fMRI. Coregistration was then performed Inhibitors,research,lifescience,medical to give information correlating functional Inhibitors,research,lifescience,medical and structural MRI data. Structural MRIs from all subjects were coaligned to generate a statistically averaged brain template. This template was used for the individual subject to whom MRI data were registered and followed by reslicing. The resulting voxel size was 2 mm × 2 mm × 2 mm. To improve the signal-to-noise ratio, the resliced fMRI data were smoothened using a Gaussian kernel of 8 mm. Image analysis The analytical method in this study was the same as that in our previous study (Yu et al. 2012). The fMRI data were estimated using the General Linear Model (GLM). For individual fMRI, Metalloexopeptidase a threshold P value of less than 0.05 (after family-wise error correction) was considered statistically significant during brain activation. For comparison between groups, a threshold P value of less than 0.001 (uncorrected) was considered statistically significant. Cluster sizes measuring 10 voxels were included for the analysis. Results The fMRI on control subjects looking at scenic pictures revealed positive sites on the parietal and visual areas (Fig. ​(Fig.1)1) with a small positive site around the central area (Fig. ​(Fig.