Conclusion: Serum CYFRA 21-1 represents a reliable diagnostic and prognostic biomarker of BTCs, especially for GBC and ICC. Disclosures: The following people have nothing to disclose: Li Huang, Wei Chen, Peiwen Liang, Wenjie Hu, Kunsong Zhang, Bin Chen, Yuyan Han, Fanyin Meng, Sharon DeMorrow, Xiaoyu Yin, Jiaming Lai, Lijian Liang Introduction: Hepatocellular carcinoma (HCC) develops on a continuum of morphological and molecular alterations in advancing chronic liver disease. FoxM1,

HKII, 8-OHdG, and iNOS have been implicated in a variety of cancers find more as markers for oncogenesis, increased metabolic activity, oxidative and nitrosative stress respectively. We hypothesize that these prooncogenic components act in concert to advance disease progression and influence tumor differentiation in HCC. Aims: To analyze immunomarkers of oncogenesis in non-dysplastic cirrhosis (NDC), liver cell change/dysplasia in cirrhosis (LCC), HCC and normal liver controls. Methodology: A progression liver tissue array 5-Fluoracil constructed from 45 subjects with cirrhosis and HCC, and 8 normal

controls was analyzed. Standard immunohistochemistry (IHC) was performed to determine levels of FOXM1, HKII, CD90, CD133, 8-OHdG, iNOS, CK7 and CK19. Staining was analyzed by Aperio Image Analysis. Fisher exact test was employed using SAS. Results: Strong positive correlations were found between various IHC stains and disease progression (Table 1). Tumor grade also correlated with CD90 hepatocyte cytoplasmic staining (CD90HS). Conclusions: Hepatocyte immunolevels of transcription factor FoxM1 and glycolytic enzyme HKII correlate with markers for hepatic cancer stem cells CD90 and CD133. Oxidative and nitrosative stress indicators medchemexpress 8-OHdG and iNOS correlate with fibrosis and disease progression markers CK7 and CK19. CD90 correlates with increasing tumor grade. These results further suggest FOXM1 and HKII play a role in promoting hepatocarcinogenesis. Disclosures: Costica Aloman – Advisory Committees or Review Panels: Gilead Sciences,

Janssen The following people have nothing to disclose: Lily Mei, Katherine M. Choi, Rajender Mulpuri, Dragana Kopanja, Hari Sreedhar, Michael J. Walsh, Ming Jin, Charmaine Stewart, Nissim Hay, Pradip Raychaudhuri, Grace Guzman Aim: Biologic features of hepatocellular carcinomas (HCCs) relate with the treatment and the prognosis. Thus, identification of biomarkers that can represent the biologic features of HCC is important. Previously, we isolated the side population (SP) cells from 2 HCC cell lines established from a single HCC nodule with histologic heterogeneity and confirmed that SP cells were more aggressive in biological features than non-SP ells in HAK-1B cells (J Gastroenterol Hepatol, 29:1092-1101, 2014).