cochinchinensis is under explored and utilized. selleck chemicals llc So, in the present study the antimicrobial potency of M. cochinchinensis seed extracts on various pathogens has been evaluated. The seeds were collected from Western Ghats, Tamilnadu, India and were identified and authenticated by renowned botanist. A voucher specimen was kept in Department of Pharmacognosy, Ultra College of Pharmacy, Madurai (Voucher specimen No: UCP/11/031). The seeds were dried in shade and powdered in a mechanical grinder. About 250 g of seed powder was macerated for one week in 1.0 L of methanol. The mass was then separated out and exhaustively macerated in

ethylacetate for another one week.5 The methanolic extract (MMC) and ethylacetate extract (EMC) were separated in rotary vacuum evaporator. The extracts thus obtained were directly used in the preliminary phytochemical screening6 and antibacterial activity. Pharmacognostical characterization was done by customary procedures.7 Photographs of different magnifications were taken with Nikon lab photo 2 microscopic unit. For normal observations selleck inhibitor bright field was used. For the study of crystals, starch grains and lignified cells, polarized light was employed. The sections were stained with toluidine blue, due rendered pink colour to the cellulose walls, blue to the lignified cells, dark green to suberin, violet to the mucilage and blue to the protein bodies. Wherever

necessary sections were also stained GPX6 with safranin and Fast-green and IKI (for starch). Magnifications of the figures are indicated by the scale-bars. Antimicrobial study was performed by disc diffusion method.8 MTCC strains like Escherichia coli MTCC 118, Proteus vulgaris

MTCC 426, Bacillus subtilis MTCC 619, Staphylococcus aureus MTCC 96, Aspergillus niger MTCC 872, Candida albicans MTCC 183 were procured from IMTECH Chandigarh. Clinical isolate Klebseilla pneumoniae M4020 was obtained from Vijay Lab, Madurai, characterized and stored. A weighed quantity of appropriate media was dissolved in sterile water and autoclaved at 121 °C for 15 min. In lukewarm condition, media was poured in Petri Plates and allowed for solidification. 24 hr old cultures were spread on to the surface of the solidified agar aseptically and carefully using a sterile L bend rod. Discs were immersed in different test concentrations (50, 100, 250 and 500 μg) of the extracts and allowed to evaporate the solvent dimethyl sulfoxide. All the discs were placed on to the surface of agar, maintaining proper distance. Plates were incubated at appropriate temperature and time in an inverted position. After incubation the zone of inhibition was measured using a metric ruler. In vertical transverse section of the seed through the hilar region these are too thick, darkly stained masses of raphe are on the either side of the hilar canal. In the median part of the seed is a spindle shaped tracheid bar flanked on the either side is loosely arranged parenchyma tissue.