Amiloride hydrochloride was obtained as gift from Panacea biotech Ltd. Chandigarh, India; Carbopol 934 P, sodium alginate, Chitosan, Eudragit RL 100, PVP K30, SCMC and EC procured from Drugs India (Hyderabad, India); sheep buccal mucosa, for determining buccoadhesive

strength and ex vivo permeation studies, was procured from a local slaughter house in Rajampet, India. All other materials used and received were of analytical grade. The buccoadhesive films were prepared by solvent casting technique with the use of “O” shaped ring placed over a glass plate as a substrate. The buccoadhesive bilayer tablets were prepared by direct compression method. This was carried out by infrared light absorption spectroscopy (IR). http://www.selleckchem.com/products/abt-199.html Infrared spectra of pure drug and

mixture of formulations were recorded by dispersion MS-275 solubility dmso of drug and mixture of formulations in suitable material (KBr) using Fourier Transform Infrared Spectrophotometer (FTIR). A base line correction was made using dried potassium bromide and then the spectra of the dried mixture of drug, formulation mixture and potassium bromide and then the spectra of the dried mixture of drug, formulation mixture and potassium bromide were recorded on FTIR. Buccal films were prepared by using HPMC alone and in combination with CP-934P, Chitosan and PVP, as shown in Table 1. Propylene glycol as a plasticizer. Ethanol was used as a solvent. The calculated amounts of polymers were dispersed in ethanol. Two hundred milligrams of Amiloride hydrochloride was incorporated in the polymeric solutions after levigation others with 30% w/w propylene glycol which served the purpose of plasticizer as well as penetration enhancer.5 The medicated gels were left overnight at room temperature to obtain clear, bubble-free gels. To prevent the evaporation of alcohol, medicated gels were filled into the vials and closed tightly by the rubber closures. The gels were casted into aluminum foil cups (4.5 cm

diameter), placed on a glass surface and allowed to dry overnight at room temperature to form a flexible film. The dried films were cut into size of 20 mm diameter, packed in aluminum foil and stored in a desiccator until further use.6 Amiloride hydrochloride buccal tablets were prepared by direct compression method. The buccal tablets were prepared by using sodium carboxy methyl cellulose (SCMC), HPMC K100, sodium alginate, Carbopol 934 P, Eudragit RL 100, PVP and ethyl cellulose (EC) as a backing layer. The above-said polymers were used in different ratios in the formulation of buccal tablets. The composition of different formulations is represented in Table 2. All the ingredients of the formulation were passed through a sieve # 85 and were blended in a glass mortar with a pestle to obtain uniform mixing.