5 ± 0 3

versus 1 0 ± 1 3 mV, p < 0 01 for post hoc test)

5 ± 0.3

versus 1.0 ± 1.3 mV, p < 0.01 for post hoc test) during both the early and late phases of SHs (Figure 4C, blue). Overall, these data indicate that SHs in V1 are due to the recruitment of GABAergic synapses. We next characterized the sub- and suprathreshold effects of noise bursts across the other layers of V1: layer 4 pyramids (L4Ps; n = 5), layer 5 pyramids (L5Ps; n = 12), and layer 6 pyramids (L6Ps; n = 7). Examples of biocytin-filled cells are shown in Figure S5A. Noise bursts elicited SHs in all recorded L6Ps, whereas they failed to elicit detectable responses in L4Ps (Figure 5A). Responses of L5Ps were heterogeneous: of 12 L5Ps, 4 were hyperpolarized, 3 were depolarized, www.selleckchem.com/products/epacadostat-incb024360.html and 5 were unaffected by sound presentation. Extracellular tetrode recordings, which have a higher sampling capability compared with in vivo whole-cell recordings, confirmed the presence of sound-driven spiking click here units in infragranular layers of V1 (see examples of simultaneously recorded units in Figure 5B). Out of 34 isolated units in infragranular layers, 8 increased firing in response to acoustic stimulation, 12 decreased firing, and 14 showed no effect on ongoing firing. Interestingly, the auditory-driven firing of these infragranular units either preceded (4/8) or accompanied the SH of L2/3Ps (Figure S5B). Thus, we asked whether infragranular neurons could trigger

sound-driven IPSPs in L2/3Ps of V1. To investigate whether L5Ps activation causes hyperpolarizing responses in L2/3Ps within the same functional column, we took advantage of the fact that in Thy1::ChR2-EYFP mice, expression of ChR2 is largely restricted to L5Ps. A 2 ms light pulse in V1 was able to cause hyperpolarizing responses in all patched L2/3Ps, and the hyperpolarizations were larger (−8.7 ± 1.3 mV) and occurred earlier (onset latency: 18.2 ± 2.4 ms) compared to SHs (n = 5 cells from 4 mice; Figure 6A). Notably, this delay corresponds

to the difference between the onset latency of SHs in L2/3Ps and that of sound-driven activation of L5Ps in V1 ( Figure 6B). More importantly, we tested the role of layer 5 in SHs of L2/3Ps by silencing activity in infragranular layers of V1 with a local puff of muscimol. We also used the injecting pipette to record very multiunit activity in layer 5 (Figure 6C). We found that the multiunit activity was silenced, confirming the neuronal inhibition (Figure 6D, gray). We then patched the overlying L2/3Ps (Figure 6D, black) to look for physiological evidence for muscimol leakage into the supragranular layers. The average Vm of the L2/3Ps was not significantly different from that recorded without muscimol injected into the deep layers (Figure 6D, left plot). We also found no change in Vmvariance in L2/3Ps after muscimol injection into the deep layers, suggesting that muscimol did not leak into the supragranular layers and affect the dynamics of spontaneous activity ( Figure 6D, right plot).

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