07 0.35 26.56-26.91 1.52 26.23-28.48 2.65 26.64-28.30 10 3 1.28 30.44-31.28 0.53 30.11-30.69 1.90 29.70-31.37 1.99 28.60-30.85 10 2 1.22 33-37-34.82 0.40 33.66-34.05 2.46 33.80-35.78 1.39 33.62-34.60 10 1 0.87 37.29-38.66 2.21 35.65-37.77 3.10 37.10-38.91 2.21 36.11-37.43 CFU/g of faeces CV c (%) Ct range CV j (%) Ct range CV c (%) Ct range CV j (%) Ct range 2 × 10 8 3.23 17.22-18.35 LY2228820 2.28 18-74-19.81 – - – - 2 × 10 7 1.33 20.60-21.15 2.53 20.57-22.02 0.75 19.54-19.88 1.21 21.65-22.27 2 × 10 6 1.89 24.08-24.97 0.91 24.13-24.62 2.37 23.51-24.85 0.70 24.15-24.60 2 × 10 5 1.15 27.23-28.38 1.40 27.02-28.45 0.57 26.40-26.79 1.46 27.04-28.69

2 × 10 4 2.20 28.28-29.75 1.98 30.13-31.80 2.58 28.00-29.90 2.10 30.7-32.31 2 × 10 3 4.40 32.20-33.77 1.62 34.61-35.96 2.07 32.00-33.22 1.80 34.48-36.45 2 × 10 2 4.38 34.61-37.78 1.76 38.04-39.37 1.64 35.35-36.56 1.92 37.34-39.03 The coefficients of variation

(CV) of the threshold cycles values (Ct) were evaluated for the C. For each CVc and CVj, the range of Ct (Ct range), which corresponds to the smallest and the highest values of the Ct found among PXD101 nmr the ten, was indicated for each dilution for both intra-and inter-assay testings. 1 Results of intra-assay testing: ten replicates of each sample were tested in one PCR run 2 Results of inter-assay testing: one replicate of each sample was tested once in each of ten different PCR runs Validation of the real time PCR assays for the analysis of faecal, feed, and environmental samples spiked with C. coli and C. jejuni Samples were checked for PCR inhibition in a separate test using a bacterial internal amplification and extraction control [34]. Inhibitors of real-time PCR were identified in 4% of the examined samples, which were consequently removed from the quantification study. The detection

limit for the quantitative real-time PCR assays in Resveratrol spiked faecal samples were 2.5 × 102 CFU of C. coli/g of faeces and 2.0 × 102 CFU of C. jejuni/g of faeces (Figure 3), similar to that of the bacteriological method. Although this assay was able to detect lower quantities Acalabrutinib datasheet between 5.0 × 101 and 2.0 × 102 CFU of Campylobacter/g of faeces, the regression curve was only linear from about 102 to 107 CFU with reaction volumes of 20 μL (Figure 3).