Stroma colour yellowish to pale PND-1186 nmr orange, 5A4, resulting from white surface and cream to yellow-ochre dots or spots; white inside. Stromata after rehydration more thickly pulvinate than dry, white with ochre-yellow perithecia; pale yellow, ochre-yellowish, dots (80–)100–160 μm diam; not changing colour in 3% KOH. Stroma anatomy: Ostioles (69–)86–111(–126) μm long, plane or projecting 14–37(–45) μm, (32–)36–54(–75) μm wide at the apex (n = 31), with clavate marginal

cells to 6 μm wide at the apex, projecting in fascicles. Perithecia selleck inhibitor (170–)200–245(–270) × (115–)130–200(–235) μm (n = 31), globose, ellipsoidal or flask-shaped, variably disposed; peridium (13–)15–21(–25) μm (n = 31) thick at the base, (6–)12–19(–22) μm (n = 31) thick at the sides; hyaline to pale yellowish. Cortical layer (15–)20–37(–56) μm (n = 30) thick, a dense t. angularis of thin- or thick-walled cells (3–)4–8(–10) × (2–)3–5(–8) μm (n = 60) in face view and in vertical section; subhyaline to pale yellowish. Cortex partly MLN2238 covered by a thin amorphous layer of more

or less compressed, undifferentiated hyphae; no differentiated hairs present. Subcortical tissue of thin-walled hyaline cells (3–)5–8(–10) × (2.5–)3.5–5.5(–7.0) μm (n = 31), mixed with hyaline hyphae (3.0–)3.5–5.5(–7.5) μm (n = 30) wide. Subperithecial tissue a t. angularis–epidermoidea of thin-walled hyaline cells (6–)8–21(–28) × (3–)7–13(–15) μm (n = 30). Stroma base of often strongly compressed, thick-walled, hyaline to pale yellowish hyphae (1.8–)2.5–5.2(–7.5) μm (n = 30) wide, extending very upwards along the sides and forming the amorphous layer on the upper surface. Asci (97–)100–116(–135) × (4.5–)5.0–6.0(–6.5) μm, stipe (11–)12–24(–31) μm long (n = 30), croziers present. Ascospores hyaline, verruculose; cells dimorphic; distal cell (3.8–)4.0–5.0(–5.5) × (3.3–)3.5–4.0(–4.3) μm, l/w (1.0–)1.1–1.3(–1.4)

(n = 30), subglobose, ellipsoidal or wedge-shaped; proximal cell (4.0–)4.5–5.5(–6.0) × (2.7–)3.0–3.5(–4.0) μm, l/w (1.1–)1.4–1.7(–1.8) (n = 30), wedge-shaped, oblong or subglobose. Cultures and anamorph: optimal growth at 15°C on all media; no growth at and above 30°C. No conidiation noted on all media. On CMD after 72 h 9–11 mm at 15°C, 5–7 mm at 25°C; mycelium covering the plate after 3 weeks at 15°C. At 15°C colony hyaline, thin, loose, circular with wavy margin, zonate; hyphae finely wavy along their length, wide, narrow secondary hyphae scant. Dense mycelial clumps formed immersed in the agar, becoming visible as whitish spots, 1–6 × 0.5–2.5 mm, in concentric zones, radially elongate, eventually turning brown. Sometimes few small brown sterile stromata appearing in irregular disposition on the colony surface. Aerial hyphae inconspicuous, more frequent at the margin. Autolytic excretions absent at 15°C, abundant at 25°C in the entire colony, minute, turning yellowish brown; coilings rare.