Thus, in our experimental setting, the simultaneous presence of different immune populations in total PBMCs assured the presence of all the required signals for B-cell differentiation and offered a faithful

representation of what is actually happening in vivo in the peripheral blood of MS patients. Our results demonstrate a fundamental difference in the outcome of either TLR7 or TLR9 stimulation of B cells 5-Fluoracil molecular weight in the context of PBMCs isolated from HDs or MS patients. Indeed, while the treatment with a TLR9 ligand induced a comparable production of both IgG and IgM in control or MS-affected individuals, we highlighted for the first time a clear deficiency in TLR7-mediated B-cell differentiation into Ig-secreting cells in MS patients. In vivo administered IFN-β is able to replenish in MS patients the low TLR7-induced Ig production to the level observed in HDs. In line with this evidence and consistent with previous findings [33], TLR7 expression was also upregulated by IFN-β both in whole PBMCs, purified B cells, and monocytes. Furthermore, three studies reported with different experimental approaches how IFN-α, another subtype of the type I IFN family

to which IFN-β belongs, exogenously provided or in situ produced by plasmacytoid DC, enhances B-cell differentiation into IgM- Venetoclax manufacturer and IgG-producing cells only in response to TLR7, but not TLR9, triggering [34-36]. We believe that in our settings

in vivo IFN-β therapy might have similar activity to what is described in vitro for IFN-α. IFN-β treatment enhances TLR7-induced B-cell responses in MS patients acting at different steps: not only on the regulation of TLR7 gene Leukotriene-A4 hydrolase expression but also on the secretion of soluble factors of key importance for B-cell differentiation, namely IL-6 and BAFF. IL-6 promotes terminal differentiation of B cells to plasma cells [23, 37] and exerts also a pronounced effect on the survival and/or Ig secretion [38]. BAFF regulates, in tandem with APRIL (a proliferation-inducing ligand), B-cell survival, differentiation and class switching, determines the size of the peripheral B-cell pool and is essential for maintenance of the peripheral B-cell repertoire and initiation of T-cell independent B-cell responses [39]. BAFF has been implicated in the development of autoimmunity in experimental settings and in several human B-cell-related autoimmune diseases, including MS [39]. Interestingly, Serafini and Aloisi in collaboration with our team also found that BAFF is expressed in EBV-infected B cells in acute MS lesions and ectopic B-cell follicles [40], highlighting the key role of this factor in B-cell activation also in the MS brain.